Diagnostic study of some Fatty acids and Antibacterial activity of Bauhinia variegate leaves extracts in some Gram positive and Gram negative bacteria

Abstract

Antibacterial activity of methanol and equeous extracts of leaves of Banhinia variegata was determined employing the disc diffusion technique, against Escherichia coli (gram negative bacteria) and Staphylococcus aureus (gram positive bacteria). Gentamicin was used as a reference standards for comparism with the extracts activities. The results were appeared with the two extracts owns antibacterial activity against the bacteria that been tested. The methalonic extract of B.variegata was showed highest zone of growth inhibition in staph. aureus was (20 mm) , while the zone of growth inhibition in E.coli was (16 mm). The activity at aqueous extract showed (18 mm) in growth inhibition in staph. aureus and (14 mm) in growth of E. coli .The methanolic extract has much more activity compared to aqueous solutions, in both bacteria, and staph that been tested. aureus was more sensitive than E.coli to both extracts.